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Proceedings Paper

The use of a handheld Raman system for virus detection
Author(s): Chunyuan Song; Jeremy D. Driskell; Ralph A. Tripp; Yiping Cui; Yiping Zhao
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Paper Abstract

The combination of surface enhanced Raman spectroscopy (SERS) with a handheld Raman system would lead to a powerful portable device for defense and security applications. The Thermo Scientific FirstDefender RM instrument is a 785-nm handheld Raman spectrometer intended for rapid field identification of unknown solid and liquid samples. Its sensitivity and effectiveness for SERS-based detection was initially confirmed by evaluating detection of 1,2-di(4- pyridyl)ethylene as a reporter molecule on a silver nanorod (AgNR) substrate, and the results are comparable to those from a confocal Bruker Raman system. As avian influenza A viruses (AIV) are recognized as an important emerging threat to public health, this portable handheld Raman spectrometer is used, for the first time, to detect and identify avian influenza A viruses using a multi-well AgNR SERS chip. The SERS spectra obtained had rich peaks which demonstrated that the instrument can be effectively used for SERS-based influenza virus detection. According to the SERS spectra, these different influenza viruses were distinguished from the negative control via the principal component analysis and by partial least squares-discriminate analysis. Together, these results show that the combination effective SERS substrates when combined with a portable Raman spectrometer provides a powerful field device for chemical and biological sensing.

Paper Details

Date Published: 4 May 2012
PDF: 7 pages
Proc. SPIE 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII, 83580I (4 May 2012); doi: 10.1117/12.918758
Show Author Affiliations
Chunyuan Song, The Univ. of Georgia (United States)
Southeast Univ. (China)
Jeremy D. Driskell, The Univ. of Georgia (United States)
Ralph A. Tripp, The Univ. of Georgia (United States)
Yiping Cui, Southeast Univ. (China)
Yiping Zhao, The Univ. of Georgia (United States)

Published in SPIE Proceedings Vol. 8358:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII
Augustus Way Fountain, Editor(s)

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