Share Email Print
cover

Proceedings Paper

Multi-wavelength resonance Raman spectroscopy of bacteria to study the effects of growth condition
Author(s): Nagapratima Kunapareddy; Jacob Grun; Robert Lunsford; David Gillis; Sergei Nikitin; Zheng Wang
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

We will examine the use of multi-wavelength UV resonance-Raman signatures to identify the effects of growth phase on different types of bacteria. Gram positive and gram-negative species, Escherichia coli, Bacillus cereus, Citrobacter koseri and Citrobacter braakii were grown to logarithmic and stationary phases in different culture media. Raman spectra of bacteria were obtained by sequential illumination of samples between 220 and 260 nm; a range which encompasses the resonance frequencies of cellular components. In addition to the information contained in the single spectrum, this two-dimensional signature contains information reflecting variations in resonance cross sections with illumination wavelength. Results of our algorithms in identifying the differences between these germs are discussed. Preliminary results indicate that growth affects the Raman signature, but not to an extent that would negate identification of the species.

Paper Details

Date Published: 5 May 2012
PDF: 7 pages
Proc. SPIE 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII, 83580B (5 May 2012); doi: 10.1117/12.918652
Show Author Affiliations
Nagapratima Kunapareddy, Research Support Instruments, Inc. (United States)
Jacob Grun, U.S. Naval Research Lab. (United States)
Robert Lunsford, U.S. Naval Research Lab. (United States)
David Gillis, U.S. Naval Research Lab. (United States)
Sergei Nikitin, Research Support Instruments, Inc. (United States)
Zheng Wang, U.S. Naval Research Lab. (United States)


Published in SPIE Proceedings Vol. 8358:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII
Augustus Way Fountain, Editor(s)

© SPIE. Terms of Use
Back to Top