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Proceedings Paper

Identification and characterization of Salmonella serotypes using DNA spectral characteristics by fourier transform infrared
Author(s): Jaya Sundaram; Bosoon Park; Arthur Hinton; Seung Chul Yoon; Kurt C. Lawrence
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Paper Abstract

Analysis of DNA samples of Salmonella serotypes were performed using FT-IR spectrometer by placing directly in contact with a diamond attenuated total reflection (ATR) crystal. Spectra were recorded from 4000 cm-1 to 525 cm-1 wavenumber with the resolution of 4 cm-1 and data spacing of 1.928 cm-1. Collected spectra were subtracted from the background spectra of empty diamond crystal surface. Principal Component Analysis (PCA) was conducted at four different spectral regions to differentiate the different serotypes of Salmonella on the basis of difference in their spectral features of DNA structure macromolecules. PCA was used to show the natural clusters in the data set and to describe the difference between the sample clusters. At the region 1800 - 1200 cm-1, PC1 distinguished 93 % and PC2 distinguished 7 % of the serotypes. Therefore, maximum classification of 100 % in total was obtained at this region. For all the Salmonella serotypes, the frequency between 1000-1150 cm-1 and 1170 -1280 cm-1 had higher loading values which showed their significant contribution in the serotype classification.

Paper Details

Date Published: 4 May 2012
PDF: 15 pages
Proc. SPIE 8369, Sensing for Agriculture and Food Quality and Safety IV, 83690O (4 May 2012); doi: 10.1117/12.918585
Show Author Affiliations
Jaya Sundaram, USDA Agricultural Research Service (United States)
Bosoon Park, USDA Agricultural Research Service (United States)
Arthur Hinton, USDA Agricultural Research Service (United States)
Seung Chul Yoon, USDA Agricultural Research Service (United States)
Kurt C. Lawrence, USDA Agricultural Research Service (United States)

Published in SPIE Proceedings Vol. 8369:
Sensing for Agriculture and Food Quality and Safety IV
Moon S. Kim; Shu-I Tu; Kuanglin Chao, Editor(s)

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