Share Email Print
cover

Proceedings Paper

In vitro quantitation of human femoral artery atherosclerosis using near-infrared Raman spectroscopy
Author(s): Ava C. Dykes; Pavlos Anastasiadis; John S. Allen; Shiv K. Sharma
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Near-infrared Raman spectroscopy has been used in vitro to identify calcified atherosclerotic plaques in human femoral arteries. Raman techniques allow for the identification of these plaques in a nondestructive manner, which may allow for the diagnosis of coronary artery disease in cardiac patients in the future. As Raman spectroscopy also reveals chemical information about the composition of the arteries, it can also be used as a prognostic tool. The in vivo detection of atherosclerotic plaques at risk for rupture in cardiac patients will enhance treatment methods while improving clinical outcomes for these procedures. Raman spectra were excited by an Invictus 785-nm NIR laser and measured with a fiber-coupled micro-Raman RXN system (Kaiser Optical Systems, Inc., Ann Arbor, MI) equipped with a 785 nm CW laser and CCD detector. Chemical mapping of arteries obtained post mortem allowed for the discrete location of atherosclerotic plaques. Raman peaks at 961 and 1073 cm-1 reveal the presence of calcium hydroxyapatite and carbonate apatite, which are known to be present in calcified plaques. By mapping the locations of these peaks the boundaries of the plaques can be precisely determined. Areas of varying degrees of calcification were also identified. Because this can be useful in determining the degree of plaque calcification and vessel stenosis, this may have a significant impact on the clinical treatment of atherosclerotic plaques in the future.

Paper Details

Date Published: 16 May 2012
PDF: 10 pages
Proc. SPIE 8367, Smart Biomedical and Physiological Sensor Technology IX, 836705 (16 May 2012); doi: 10.1117/12.918520
Show Author Affiliations
Ava C. Dykes, Hawai'i Institute of Geophysics and Planetology (United States)
Pavlos Anastasiadis, Univ. of Hawai'i at Manoa (United States)
John S. Allen, Univ. of Hawai'i at Manoa (United States)
Shiv K. Sharma, Hawai'i Institute of Geophysics and Planetology (United States)


Published in SPIE Proceedings Vol. 8367:
Smart Biomedical and Physiological Sensor Technology IX
Brian M. Cullum; Eric S. McLamore, Editor(s)

© SPIE. Terms of Use
Back to Top