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Proceedings Paper

Testing and comparison of the coating materials for immunosensors on QCM
Author(s): Ali Oztuna; Hasan Nazir
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Paper Abstract

In immunoassay based biosensors development studies polymers, as a matrix, and thiol, amine and aldehyde derivative compounds, as a antibody linker, are to be experimented. Aim of this study is to test amine and acetate functional group containing derivatives in liquid phase in order to develop an antibody immobilization strategy for Quartz Crystal Microbalance (QCM) system. In our study, 4-aminothiophenol (4-AT), carboxylated-PVC (PVC-COOH) and aminated- PVC (PVC-NH2) compared with each other as a coating material. Surface of the coated AT-cut gold crystals were characterized with Fourier Transform Infrared spectrometry (FTIR) and Scanning Electron Microscobe (SEM) and tested in a Bacillus anthracis (GenBank: GQ375871) spores immunoassay model system. Subsequently, a series of SEM micrographs were taken again in order to investigate surface morphology and show the presence of the B. anthracis spores on the sensor surface. When experimental results and SEM images were evaluated together, it was suggested that with the synthesis of PVC like open-chained polymers, containing -NH2 and -SH functional groups, B. anthracis spore detection can be accomplished on QCM without requiring complicated immobilization procedures and expensive preliminary preparations.

Paper Details

Date Published: 5 May 2012
PDF: 9 pages
Proc. SPIE 8358, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII, 83580J (5 May 2012); doi: 10.1117/12.918450
Show Author Affiliations
Ali Oztuna, Gulhane Military Medical Academy (Turkey)
Hasan Nazir, Ankara Univ. (Turkey)


Published in SPIE Proceedings Vol. 8358:
Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII
Augustus Way Fountain, Editor(s)

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