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Proceedings Paper

Shining new light on optogenetics
Author(s): Ling Gu; Kamal Dhakal; Ting Li; Samarendra Mohanty
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Paper Abstract

Since development of optogenetic stimulation paradigm, there has been several attempts to red shift the excitation maximum of the efficient blue-sensitive opsins. While there has been some success at the cost of altered light-activation kinetics, near-infrared optogenetic probe will be ideal for in-depth cell-specific stimulation of excitable cells in an organ. However, single-photon near-infrared optogenetics based stimulation will still limit precise probing and modulation of in-vivo neural circuits. In contrast, by virtue of non-linear nature of ultrafast light-matter interaction, high spatial precision in optogenetic activation can be achieved in addition to inherent cellular specificity and temporal resolution provided by the opsins. Here, we report use of non-linear optogenetics for stimulation of neurons in-vivo in mouse models. Advantage of using non-linear optogenetics for probing neuronal circuitry is discussed. Further, effectiveness of the non-diffracting optogenetic Bessel beam over classical Gaussian beam in a layered mouse-brain geometry is demonstrated using Monte Carlo (MC) simulation. This is corroborated by electrophysiological measurements in in-vivo mouse models. The large propagation distance, characteristics of Bessel beam is better suited for in-depth single as well as two-photon optogenetic stimulation.

Paper Details

Date Published: 17 February 2012
PDF: 8 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 820764 (17 February 2012); doi: 10.1117/12.916750
Show Author Affiliations
Ling Gu, The Univ. of Texas at Arlington (United States)
Univ. of Electronic Science and Technology of China (China)
Kamal Dhakal, The Univ. of Texas at Arlington (United States)
Univ. of Electronic Science and Technology of China (China)
Ting Li, The Univ. of Texas at Arlington (United States)
Univ. of Electronic Science and Technology of China (China)
Samarendra Mohanty, The Univ. of Texas at Arlington (United States)
Univ. of Electronic Science and Technology of China (China)


Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg; Melissa J. Suter; Brian Jet-Fei Wong; Justus F. Ilgner; Stephen Lam; Bodo E. Knudsen; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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