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Proceedings Paper

Real-time detection of α-thrombin binding to single-strand DNA aptamers by a highly sensitive Si-based waveguide SPR biosensor
Author(s): Chi-Chieh Huang; Hsin-Feng Hsu; Sz-Hau Chen; Kun-Yu Tsai; Yang-Tung Huang; Chih-Sheng Lin; Shih-Hsin Hsu
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Paper Abstract

αIn this paper, real-time characterization of α-thrombin binding to single-strand DNA (ssDNA) aptamers by novel Si-based waveguide SPR biosensors has been investigated. The gold nanoparticles (AuNPs) modified with anti-thrombin antibodies were employed to bind with α-thrombin via strong antibody/antigen affinity for SPR signal amplification. The detection limit of 1 pM for -thrombin detection was achieved.

Paper Details

Date Published: 31 January 2012
PDF: 5 pages
Proc. SPIE 8351, Third Asia Pacific Optical Sensors Conference, 83512R (31 January 2012); doi: 10.1117/12.915947
Show Author Affiliations
Chi-Chieh Huang, National Chiao Tung Univ. (Taiwan)
Hsin-Feng Hsu, National Chiao Tung Univ. (Taiwan)
Sz-Hau Chen, National Chiao Tung Univ. (Taiwan)
Kun-Yu Tsai, National Chiao Tung Univ. (Taiwan)
Yang-Tung Huang, National Chiao Tung Univ. (Taiwan)
Chih-Sheng Lin, National Chiao Tung Univ. (Taiwan)
Shih-Hsin Hsu, National Chiao Tung Univ. (Taiwan)


Published in SPIE Proceedings Vol. 8351:
Third Asia Pacific Optical Sensors Conference
John Canning; Gangding Peng, Editor(s)

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