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Proceedings Paper

Advances in super-resolution technology and application in biomedical research
Author(s): Christopher B. O'Connell; Stephen T. Ross
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Paper Abstract

The diffraction limit of the conventional light microscope establishes a barrier that limits resolution and prevents observation of fine structural details within biological specimens. A number of commercially available systems now enable researchers to beat diffraction and achieve up to ten-fold improvements in resolution. These super resolution systems generally rely on one of two strategies. They either add optical elements in order to overcome the diffraction limit or they implement computational power and fitting algorithms to circumvent it. We have now entered the next phase in the development of super resolution systems, where probes, hardware, and software are gaining the refinements necessary to facilitate their application to a range of biological problems. Here, we highlight the recent developments in these areas for two types of super resolution imaging systems, structured illumination microscopy (SIM) and stochastic optical reconstruction microscopy (STORM). These improvements are promoting the fast acquisition and processing speeds needed for live cell imaging beyond the diffraction limit.

Paper Details

Date Published: 13 February 2012
PDF: 6 pages
Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280U (13 February 2012); doi: 10.1117/12.915577
Show Author Affiliations
Christopher B. O'Connell, Nikon Instruments Inc. (United States)
Stephen T. Ross, Nikon Instruments Inc. (United States)


Published in SPIE Proceedings Vol. 8228:
Single Molecule Spectroscopy and Superresolution Imaging V
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling; Ingo Gregor, Editor(s)

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