Share Email Print
cover

Proceedings Paper

Time variation of adipose tissue refractive index under photodynamic treatment: in vitro study using OCT
Author(s): Irina Yu. Yanina; Natalia A. Trunina; Valery V. Tuchin
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Time variation of the adipose tissue refractive index under photodynamic treatment was studied using OCT. Fat tissue slices 200-500 μm thick were used in in vitro experiments. To stain the fat tissue we used water-ethanol solutions of indocyanine green (ICG) and brilliant green (BG) with the concentration 1 mg/ml and 6 mg/ml, respectively. The CW laser diode (VD-VII DPSS, 808 nm) and the dental diode irradiator Ultra Lume Led 5 (442 and 597 nm) were used for irradiation of tissue slices. The irradiation time was 5 min for the laser and 15 min for the diode lamp. The experiments were carried out at room temperature. It was discovered the immersion optical clearing of fat tissue slices due to fat cell lipolysis under photodynamic treatment. Released cell content works as an immersion agent, thus the relative refractive index of tissue scatterers decreasing with the time elapsed after the treatment. These data support the hypothesis that photodynamic treatment induces fat cell lipolysis for some period after treatment.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8222, Dynamics and Fluctuations in Biomedical Photonics IX, 82221G (9 February 2012); doi: 10.1117/12.915485
Show Author Affiliations
Irina Yu. Yanina, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Natalia A. Trunina, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Valery V. Tuchin, N.G. Chernyshevsky Saratov State Univ. (Russian Federation)
Institute of Precise Mechanics and Control (Russian Federation)
Univ. of Oulu (Finland)


Published in SPIE Proceedings Vol. 8222:
Dynamics and Fluctuations in Biomedical Photonics IX
Valery V. Tuchin; Donald D. Duncan; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

© SPIE. Terms of Use
Back to Top