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Proceedings Paper

Measurement of kidney stone formation in the rat model using micro-computed tomography
Author(s): Joseph U. Umoh; Vasek Pitelka; Harvey A. Goldberg; David W. Holdsworth
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Paper Abstract

Kidney stones were induced in 5 rats by treating them with 1% ethylene glycol and 1% ammonium chloride through free drinking water for six weeks. The animals were anesthetized and imaged in vivo before the treatment at week 0, to obtain baseline data, then at weeks 2 and 6 to monitor the kidney stone formation. Micro-CT imaging was performed with x-ray tube voltage of 90 kV and a current of 40 mA. At week 2, kidney stone formation was observed. A micro-computed tomography methodology of estimating the volume and hydroxyapatite-equivalent mineral content of the kidney stone is presented. It determines the threshold CT number (390 HU) that separates the kidney stone from the tissue. The mean volume of the stones in the 10 kidneys significantly increased from 3.81±0.72 mm3 at week 2 to 23.96±9.12 mm3 at week 6 (p<0.05, r2=0.34). Measurement precision error was about 4%. This method allows analysis of the kidney stone formation to be carried out in vivo, with fewer experimental animals compared with other ex vivo methods, in which animals are sacrificed. It is precise, accurate, non-destructive, and could be used in pre-clinical research to study the formation of kidney stones in live small animals.

Paper Details

Date Published: 16 April 2012
PDF: 8 pages
Proc. SPIE 8317, Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging, 831721 (16 April 2012); doi: 10.1117/12.913346
Show Author Affiliations
Joseph U. Umoh, Pre-Clinical Imaging Research Ctr., Robarts Research Institute, The Univ. of Western Ontario (Canada)
Vasek Pitelka, CIHR Group in Skeletal Development and Remodelling, The Univ. of Western Ontario (Canada)
Harvey A. Goldberg, CIHR Group in Skeletal Development and Remodelling, The Univ. of Western Ontario (Canada)
David W. Holdsworth, Pre-Clinical Imaging Research Ctr., Robarts Research Institute, The Univ. of Western Ontario (Canada)


Published in SPIE Proceedings Vol. 8317:
Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging
Robert C. Molthen; John B. Weaver, Editor(s)

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