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Proceedings Paper

Low level laser therapy reduces oxidative stress in cortical neurons in vitro
Author(s): Ying-Ying Huang; Clark E. Tedford; Thomas McCarthy; Michael R. Hamblin
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Paper Abstract

It is accepted that the mechanisms of low level laser therapy (LLLT) involves photons that are absorbed in the mitochondria of cells and lead to increase of mitochondrial metabolism resulting in more electron transport, increase of mitochondrial membrane potential, and more ATP production. Intracellular calcium changes are seen that correlate with mitochondrial stimulation. The situation with two other intermediates is more complex however: reactive oxygen species (ROS) and nitric oxide (NO). Evidence exists that low levels of ROS are produced by LLLT in normal cells that can be beneficial by (for instance) activating NF-kB. However high fluences of light can produce large amounts of ROS that can damage the cells. In oxidatively stressed cells the situation may be different. We exposed primary cultured cortical neurons to hydrogen peroxide (H2O2) or cobalt chloride (CoCl2) oxidative insults in the presence or absence of LLLT (810-nm laser at 0.3 or 3 J/cm2). Cell viability of cortical neurons was determined by lactate dehydrogenase assay. ROS in neurons was detected using an ROS probe, MitoRox with confocal microscopy. Results showed that LLLT dose-dependently reversed ROS production and protected cortical neurons against H2O2 or CoCl2 induced oxidative injury in cultured cortical neurons. Conclusion: LLLT can protect cortical neurons against oxidative stress by reversing the levels of ROS.

Paper Details

Date Published: 10 March 2012
PDF: 8 pages
Proc. SPIE 8211, Mechanisms for Low-Light Therapy VII, 821103 (10 March 2012); doi: 10.1117/12.912948
Show Author Affiliations
Ying-Ying Huang, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Guangxi Medical Univ. (China)
Clark E. Tedford, PhotoThera, Inc. (United States)
Thomas McCarthy, PhotoThera, Inc. (United States)
Michael R. Hamblin, Wellman Ctr. for Photomedicine, Massachusetts General Hospital (United States)
Harvard Medical School (United States)
Harvard-MIT Division of Health Sciences and Technology (United States)


Published in SPIE Proceedings Vol. 8211:
Mechanisms for Low-Light Therapy VII
Michael R. Hamblin; Juanita Anders; James D. Carroll, Editor(s)

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