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Proceedings Paper

In vivo deep tissue fluorescence imaging of the murine small intestine and colon
Author(s): Viera Crosignani; Alexander Dvornikov; Jose S. Aguilar; Chiara Stringari; Roberts Edwards; Williams Mantulin; Enrico Gratton
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Paper Abstract

Recently we described a novel technical approach with enhanced fluorescence detection capabilities in two-photon microscopy that achieves deep tissue imaging, while maintaining micron resolution. This technique was applied to in vivo imaging of murine small intestine and colon. Individuals with Inflammatory Bowel Disease (IBD), commonly presenting as Crohn's disease or Ulcerative Colitis, are at increased risk for developing colorectal cancer. We have developed a Giα2 gene knock out mouse IBD model that develops colitis and colon cancer. The challenge is to study the disease in the whole animal, while maintaining high resolution imaging at millimeter depth. In the Giα2-/- mice, we have been successful in imaging Lgr5-GFP positive stem cell reporters that are found in crypts of niche structures, as well as deeper structures, in the small intestine and colon at depths greater than 1mm. In parallel with these in vivo deep tissue imaging experiments, we have also pursued autofluorescence FLIM imaging of the colon and small intestine-at more shallow depths (roughly 160μm)- on commercial two photon microscopes with excellent structural correlation (in overlapping tissue regions) between the different technologies.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822609 (9 February 2012); doi: 10.1117/12.912841
Show Author Affiliations
Viera Crosignani, Univ. of California, Irvine (United States)
Alexander Dvornikov, Univ. of California, Irvine (United States)
Jose S. Aguilar, Univ. of California, Irvine (United States)
Chiara Stringari, Univ. of California, Irvine (United States)
Roberts Edwards, Univ. of California, Irvine (United States)
Williams Mantulin, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)
Enrico Gratton, Univ. of California, Irvine (United States)
Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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