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Proceedings Paper

Latest advances in commercially available STED microscopy
Author(s): Wernher Fouquet; Arnold Giske
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Paper Abstract

STimulated Emission Depletion (STED) microscopy enables imaging of biological samples combining significantly improved optical resolution with all benefits of confocal microscopy. Especially, by combining multi-channel image acquisition with high spatial resolution opens up a new understanding of co-localization experiments on nanoscales. Such a microscope provides new insights in various fields of biology, such as cell and membrane biology, neurobiology and physiology. We present new developments and a variety of biological examples for STED microscopy, showing structural details on scales well below 70nm and give an overview of possible field of applications, mainly focused on live cell imaging.

Paper Details

Date Published: 13 February 2012
PDF: 6 pages
Proc. SPIE 8228, Single Molecule Spectroscopy and Superresolution Imaging V, 82280W (13 February 2012); doi: 10.1117/12.912751
Show Author Affiliations
Wernher Fouquet, Leica Microsystems CMS GmbH (Germany)
Arnold Giske, Leica Microsystems CMS GmbH (Germany)

Published in SPIE Proceedings Vol. 8228:
Single Molecule Spectroscopy and Superresolution Imaging V
Jörg Enderlein; Zygmunt Karol Gryczynski; Rainer Erdmann; Felix Koberling; Ingo Gregor, Editor(s)

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