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Proceedings Paper

Raman microspectroscopy of algal lipid bodies: β-carotene as a volume sensor
Author(s): Zdenek Pilát; Silvie Bernatová; Jan Ježek; Mojmir Šery; Ota Samek; Pavel Zemánek; Ladislav Nedbal; Martin Trtílek
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Paper Abstract

Advanced optical instruments are useful for analysis and manipulation of individual living cells and their internal structures. We have employed Raman microspectroscopic analysis for assessment of algal lipid body (LB) volume in vivo. Some algae contain β-carotene in high amounts in their LBs, including strains which are considered useful in biotechnology for lipid and pigment production. We have detected proportionality between the Raman vibrations of β-carotene and the LB volume. This finding may allow fast acquisition of LB volume approximation valuable e.g. for Raman microspectroscopy assisted cell sorting. We combine optical manipulation and analysis on a microfluidic platform in order to achieve fast, effective, and non-invasive sorting based on spectroscopic features of the individual living cells. The resultant apparatus could find its use in demanding biotechnological applications such as selection of rare natural mutants or artificially modified cells resulting from genetic manipulations.

Paper Details

Date Published: 11 October 2011
PDF: 7 pages
Proc. SPIE 8306, Photonics, Devices, and Systems V, 83060L (11 October 2011); doi: 10.1117/12.912264
Show Author Affiliations
Zdenek Pilát, Institute of Scientific Instruments (Czech Republic)
Silvie Bernatová, Institute of Scientific Instruments (Czech Republic)
Jan Ježek, Institute of Scientific Instruments (Czech Republic)
Mojmir Šery, Institute of Scientific Instruments (Czech Republic)
Ota Samek, Institute of Scientific Instruments (Czech Republic)
Pavel Zemánek, Institute of Scientific Instruments (Czech Republic)
Ladislav Nedbal, Global Change Research Ctr. (Czech Republic)
Martin Trtílek, Photon Systems Instruments (Czech Republic)

Published in SPIE Proceedings Vol. 8306:
Photonics, Devices, and Systems V
Pavel Tománek; Dagmar Senderáková; Petr Páta, Editor(s)

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