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Proceedings Paper

Kynetic resazurin assay (KRA) for bacterial quantification of foodborne pathogens
Author(s): Yaxal Arenas; Arkady Mandel; Lothar Lilge
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Paper Abstract

Fast detection of bacterial concentrations is important for the food industry and for healthcare. Early detection of infections and appropriate treatment is essential since, the delay of treatments for bacterial infections tends to be associated with higher mortality rates. In the food industry and in healthcare, standard procedures require the count of colony-forming units in order to quantify bacterial concentrations, however, this method is time consuming and reports require three days to be completed. An alternative is metabolic-colorimetric assays which provide time efficient in vitro bacterial concentrations. A colorimetric assay based on Resazurin was developed as a time kinetic assay (KRA) suitable for bacterial concentration measurements. An optimization was performed by finding excitation and emission wavelengths for fluorescent acquisition. A comparison of two non-related bacteria, foodborne pathogens Escherichia coli and Listeria monocytogenes, was performed in 96 well plates. A metabolic and clonogenic dependence was established for fluorescent kinetic signals.

Paper Details

Date Published: 14 February 2012
PDF: 5 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82252K (14 February 2012); doi: 10.1117/12.912177
Show Author Affiliations
Yaxal Arenas, Theralase, Inc. (Canada)
Arkady Mandel, Theralase, Inc. (Canada)
Lothar Lilge, Ontario Cancer Institute, Univ. of Toronto (Canada)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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