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Proceedings Paper

Nano particles insertion into individual mammalian cells using optical tweezers
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Paper Abstract

Transfection is the process of introducing DNA into cells so that the introduced DNA will function and produce proteins. This technique is useful to study the function of various DNA sequences and in the future may lead to gene therapy for curing genetic diseases. Currently, a number of techniques are available for both population and individual cells transfection. Although individual cells transfection is less commonly used than the population transfection, it has benefits because it allows controlled single cell analysis. In this paper, we present a new laser assisted transfection method for individual cells. In this technique, two lasers are used to perform the transfection procedure and third laser is used to detect the position of DNA coated nanoparticle which is inserted in the cell. This technique has relatively high transfection efficiency and good post-transfection cell viability.

Paper Details

Date Published: 30 January 2012
PDF: 6 pages
Proc. SPIE 8247, Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XII, 824706 (30 January 2012); doi: 10.1117/12.912056
Show Author Affiliations
Muhammad Waleed, Gwangju Institute of Science and Technology (Korea, Republic of)
Jung-Dae Kim, Gwangju Institute of Science and Technology (Korea, Republic of)
Yong-Gu Lee, Gwangju Institute of Science and Technology (Korea, Republic of)


Published in SPIE Proceedings Vol. 8247:
Frontiers in Ultrafast Optics: Biomedical, Scientific, and Industrial Applications XII
Alexander Heisterkamp; Michel Meunier; Stefan Nolte, Editor(s)

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