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Proceedings Paper

Adaptive optics confocal microscopy using fluorescent protein guide-stars for brain tissue imaging
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Paper Abstract

Optical aberrations due to the inhomogeneous refractive index of tissue degrade the resolution and brightness of images in deep tissue imaging. We introduce a direct wavefront sensing method using cellular structures labeled with fluorescent proteins in tissues as guide-stars. As a non-invasive and high-speed method, it generalizes the direct wavefront sensing method for adaptive optics microscopy. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. The confocal images with and without correction are collected. The results show increased image contrast and 3X improvement in the signal intensity for fixed mouse tissues at a depth of 70 μm. The images of the dendrite and spines are much clearer after correction with improved contrast. The Strehl ratio is improved from 0.29 to 0.96, a significant 3.3X improvement.

Paper Details

Date Published: 15 February 2012
PDF: 6 pages
Proc. SPIE 8253, MEMS Adaptive Optics VI, 82530M (15 February 2012); doi: 10.1117/12.911956
Show Author Affiliations
Xiaodong Tao, W.M. Keck Ctr. for Adaptive Optical Microscopy, Univ. of California, Santa Cruz (United States)
Oscar Azucena, W.M. Keck Ctr. for Adaptive Optical Microscopy, Univ. of California, Santa Cruz (United States)
Min Fu, Univ. of California, Santa Cruz (United States)
Yi Zuo, Univ. of California, Santa Cruz (United States)
Diana C. Chen, Lawrence Livermore National Lab. (United States)
Joel Kubby, W.M. Keck Ctr. for Adaptive Optical Microscopy, Univ. of California, Santa Cruz (United States)


Published in SPIE Proceedings Vol. 8253:
MEMS Adaptive Optics VI
Scot S. Olivier; Thomas G. Bifano; Joel Kubby, Editor(s)

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