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Proceedings Paper

Long-range mechanical force in colony branching and tumor invasion
Author(s): Chin-Lin Guo; Mingxing Ouyang; Jiun-Yann Yu; Andrew Price; Jordan Maslov
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Paper Abstract

The most concerned factors for cancer prognosis are tumor invasion and metastasis. The patterns of tumor invasion can be characterized as random infiltration to surrounding extracellular matrix (ECM) or formation of long-range path for collective migration. Recent studies indicate that mechanical force plays an important role in tumor infiltration and collective migration. However, how tumor colonies develop mechanical interactions with each other to initiate various invasion patterns is unclear. Using a micro-patterning technique, we partition cells into clusters to mimic tumor colonies and quantitatively induce colony-ECM interactions. We find that pre-malignant epithelial cells, in response to concentrations of type I collagen in ECM ([COL]), develop various branching patterns resembling those observed in tumor invasion. In contrast with conventional thought, these patterns require long-range (~ 600 μm) transmission of traction force, but not biochemical factors. At low [COL], cell colonies synergistically develop pairwise and directed branching mimicking the formation of long-range path. By contrast, at high [COL] or high colony density, cell colonies develop random branching and scattering patterns independent of each other. Our results suggest that tumor colonies might select different invasive patterns depending on their interactions with each other and with the ECM.

Paper Details

Date Published: 12 September 2011
PDF: 8 pages
Proc. SPIE 8099, Biosensing and Nanomedicine IV, 809903 (12 September 2011); doi: 10.1117/12.911904
Show Author Affiliations
Chin-Lin Guo, California Institute of Technology (United States)
Mingxing Ouyang, California Institute of Technology (United States)
Jiun-Yann Yu, California Institute of Technology (United States)
Andrew Price, California Institute of Technology (United States)
Jordan Maslov, California Institute of Technology (United States)

Published in SPIE Proceedings Vol. 8099:
Biosensing and Nanomedicine IV
Hooman Mohseni; Massoud H. Agahi; Manijeh Razeghi, Editor(s)

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