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Proceedings Paper

A cost-effective analog method to produce time-gated luminescence images
Author(s): Robert C. Leif; Sean Yang; Yiqing Lu; Dayong Jin; Stephen Chambers
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Paper Abstract

Time-gated luminescence images were obtained by analog summation of a series of sequential images that were obtained with a cooled modified interline CCD camera, and a fluorescence microscope modified to use a UV LED for illumination. The interline CCD camera obtains an analog sum of a multi-frame image by not reading out the storage line after each frame is acquired; instead, the charges from the acquisition pixels are transferred to the storage pixels, which adds them to those previously stored; subsequently, the sum of the images is readout from the storage pixels and digitized. The length of the exposure is limited by the capacity of the storage pixels and the rate of generation of background (noise). Previously, the quality of the images obtained with the room temperature camera was degraded by the buildup of thermal noise. The interline transfer, electronically shuttered, cooled astronomy CCD camera, which was modified for analog summation rapidly produced low noise images; yet permitted long exposures. The past problems with lanthanide dyes of low extinction coefficients and equipment cost have now been solved.

Paper Details

Date Published: 9 February 2012
PDF: 8 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82250Z (9 February 2012); doi: 10.1117/12.911853
Show Author Affiliations
Robert C. Leif, Newport Instruments (United States)
Sean Yang, Newport Instruments (United States)
Phoenix Flow Systems Inc. (United States)
Yiqing Lu, Macquarie Univ. (Australia)
Tsinghua Univ. (China)
Dayong Jin, Macquarie Univ. (Australia)
Stephen Chambers, Artemis CCD Ltd. (United Kingdom)

Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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