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Proceedings Paper

Impact of nanosecond pulsed electric fields on primary hippocampal neurons
Author(s): Caleb C. Roth; Jason A. Payne; Marjorie A. Kuipers; Gary L. Thompson; Gerald J. Wilmink; Bennett L. Ibey
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Paper Abstract

Cellular exposure to nanosecond pulsed electric fields (nsPEF) are believed to cause immediate creation of nanopores in the plasma membrane. These nanopores enable passage of small ions, but remain impermeable to larger molecules like propidium iodide. Previous work has shown that nanopores are stable for minutes after exposure, suggesting that formation of nanopores in excitable cells could lead to prolonged action potential inhibition. Previously, we measured the formation of nanopores in neuroblastoma cells by measuring the influx of extracellular calcium by preloading cells with Calcium Green-AM. In this work, we explored the impact of changing the width of a single nsPEF, at constant amplitude, on uptake of extracellular calcium ions by primary hippocampal neurons (PHN). Calcium Green was again used to measure the influx of extracellular calcium and FM1-43 was used to monitor changes in membrane conformation. The observed thresholds for nanopore formation in PHN by nsPEF were comparable to those measured in neuroblastoma. This work is the first study of nsPEF effects on PHN and strongly suggests that neurological inhibition by nanosecond electrical pulses is highly likely at doses well below irreversible damage.

Paper Details

Date Published: 14 March 2012
PDF: 8 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 820763 (14 March 2012); doi: 10.1117/12.911802
Show Author Affiliations
Caleb C. Roth, General Dynamics Information Technology (United States)
Jason A. Payne, Air Force Research Lab. (United States)
Marjorie A. Kuipers, Air Force Research Lab.
Gary L. Thompson, National Research Council (United States)
Gerald J. Wilmink, Air Force Research Lab. (United States)
Bennett L. Ibey, Air Force Research Lab. (United States)

Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory M.D.; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg M.D.; Melissa J. Suter; Brian Jet-Fei Wong M.D.; Justus F. Ilgner M.D.; Stephen Lam; Bodo E. Knudsen M.D.; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney M.D.; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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