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Proceedings Paper

Quantitative tracking of tumor cells in phase-contrast microscopy exploiting halo artifact pattern
Author(s): Mi-Sun Kang; Soo-Min Song; Hana Lee; Myoung-Hee Kim
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Paper Abstract

Tumor cell morphology is closely related to its invasiveness characteristics and migratory behaviors. An invasive tumor cell has a highly irregular shape, whereas a spherical cell is non-metastatic. Thus, quantitative analysis of cell features is crucial to determine tumor malignancy or to test the efficacy of anticancer treatment. We use phase-contrast microscopy to analyze single cell morphology and to monitor its change because it enables observation of long-term activity of living cells without photobleaching and phototoxicity, which is common in other fluorescence-labeled microscopy. Despite this advantage, there are image-level drawbacks to phase-contrast microscopy, such as local light effect and contrast interference ring, among others. Thus, we first applied a local filter to compensate for non-uniform illumination. Then, we used intensity distribution information to detect the cell boundary. In phase-contrast microscopy images, the cell normally appears as a dark region surrounded by a bright halo. As the halo artifact around the cell body is minimal and has an asymmetric diffusion pattern, we calculated the cross-sectional plane that intersected the center of each cell and was orthogonal to the first principal axis. Then, we extracted the dark cell region by level set. However, a dense population of cultured cells still rendered single-cell analysis difficult. Finally, we measured roundness and size to classify tumor cells into malignant and benign groups. We validated segmentation accuracy by comparing our findings with manually obtained results.

Paper Details

Date Published: 16 April 2012
PDF: 9 pages
Proc. SPIE 8317, Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging, 83171X (16 April 2012); doi: 10.1117/12.911683
Show Author Affiliations
Mi-Sun Kang, Ewha Womans Univ. (Korea, Republic of)
Soo-Min Song, Ewha Womans Univ. (Korea, Republic of)
Hana Lee, Ewha Womans Univ. (Korea, Republic of)
Myoung-Hee Kim, Ewha Womans Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 8317:
Medical Imaging 2012: Biomedical Applications in Molecular, Structural, and Functional Imaging
Robert C. Molthen; John B. Weaver, Editor(s)

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