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Proceedings Paper

Second harmonic generation microscopy differentiates collagen type I and type III in COPD
Author(s): Masaru Suzuki; Damian Kayra; W. Mark Elliott; James C. Hogg; Thomas Abraham
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Paper Abstract

The structural remodeling of extracellular matrix proteins in peripheral lung region is an important feature in chronic obstructive pulmonary disease (COPD). Multiphoton microscopy is capable of inducing specific second harmonic generation (SHG) signal from non-centrosymmetric structural proteins such as fibrillar collagens. In this study, SHG microscopy was used to examine structural remodeling of the fibrillar collagens in human lungs undergoing emphysematous destruction (n=2). The SHG signals originating from these diseased lung thin sections from base to apex (n=16) were captured simultaneously in both forward and backward directions. We found that the SHG images detected in the forward direction showed well-developed and well-structured thick collagen fibers while the SHG images detected in the backward direction showed striking different morphological features which included the diffused pattern of forward detected structures plus other forms of collagen structures. Comparison of these images with the wellestablished immunohistochemical staining indicated that the structures detected in the forward direction are primarily the thick collagen type I fibers and the structures identified in the backward direction are diffusive structures of forward detected collagen type I plus collagen type III. In conclusion, we here demonstrate the feasibility of SHG microscopy in differentiating fibrillar collagen subtypes and understanding their remodeling in diseased lung tissues.

Paper Details

Date Published: 9 February 2012
PDF: 9 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82263F (9 February 2012); doi: 10.1117/12.910815
Show Author Affiliations
Masaru Suzuki, The Univ. of British Columbia James Hogg Research Ctr. (Canada)
Hokkaido Univ. School of Medicine (Japan)
Damian Kayra, The Univ. of British Columbia James Hogg Research Ctr. (Canada)
W. Mark Elliott, The Univ. of British Columbia James Hogg Research Ctr. (Canada)
James C. Hogg, The Univ. of British Columbia James Hogg Research Ctr. (Canada)
Thomas Abraham, The Univ. of British Columbia James Hogg Research Ctr. (Canada)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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