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Proceedings Paper

Investigating the effects of terahertz radiation on Bacillus subtilis
Author(s): Jillian P. Giles; Brittany J. Raitt; Cecil S. Joseph; Mark E. Hines; Robert H. Giles
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Paper Abstract

Medical and security sensing applications of Terahertz (THz) imaging are currently being developed. As a result, there is a need to further investigate the effects of THz radiation on biological systems. In this study, a 94 GHz mechanically tuned Gunn Oscillator was used to irradiate Bacillus subtilis at 94 GHz. The bacteria were cultured in trypticase soy broth (TSB) and placed in polystyrene 96 well plates. The samples where irradiated during the exponential growth phase for 1, 2, and 24 hours. Both the experimental and control plates were kept at room temperature (~25°C) and were monitored for the duration of the experiment using thermocouples interfaced with a computer via Labview software. By evaluating the absorption of each well at 600nm immediately before and after irradiation, the population density within each well was assessed. Following this, the metabolic activity of each well was measured after irradiation by adding tetrazolium dye, XTT, to the wells and evaluating the absorption of each well at 490nm after 2 hours of incubation.

Paper Details

Date Published: 9 February 2012
PDF: 9 pages
Proc. SPIE 8222, Dynamics and Fluctuations in Biomedical Photonics IX, 822213 (9 February 2012); doi: 10.1117/12.910724
Show Author Affiliations
Jillian P. Giles, Univ. of Massachusetts Lowell (United States)
Brittany J. Raitt, Univ. of Massachusetts Lowell (United States)
Cecil S. Joseph, Univ. of Massachusetts Lowell (United States)
Mark E. Hines, Univ. of Massachusetts Lowell (United States)
Robert H. Giles, Univ. of Massachusetts Lowell (United States)


Published in SPIE Proceedings Vol. 8222:
Dynamics and Fluctuations in Biomedical Photonics IX
Valery V. Tuchin; Donald D. Duncan; Kirill V. Larin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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