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Proceedings Paper

Controlling cell-material interactions using coatings with advanced polymer architectures
Author(s): Peter Koegler; Paul Pasic; Graham Johnson; Penny Bean; Guenter Lorenz; Laurence Meagher; Helmut Thissen
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Paper Abstract

Polymeric coatings which allow the effective control of biointerfacial interactions and cellular responses are of increasing interest in a range of biomedical applications in vitro and in vivo such as cell culture tools, biosensors and implantable medical devices. A variety of coating strategies have been developed to gain control over cell-surface interactions but many of them are limited with respect to their function and transferability between different substrate materials. Here, our aim was to establish an easily transferable coating that reduces non-specific cell-surface interactions to a minimum while at the same time presenting functional groups which allow for the subsequent immobilisation of bioactive signals. To achieve this, we have applied an allylamine plasma polymer coating followed by the covalent immobilisation of a macro-initiator providing iniferter functional groups. Subsequent controlled free radical graft polymerisation using the monomers acrylamide and acrylic acid in different molar ratios resulted in highly uniform polymer coatings. Non-specific cell attachment was significantly reduced on coatings representing molar ratios of less than 10% acylic acid. At the same time, we have demonstrated the suitability of these coatings for the subsequent covalent binding of bioactive compounds carrying amine functional groups using the label 2,2,2-trifluoroethylamine. Successful surface modifications were confirmed by X-ray photoelectron spectroscopy (XPS) and profilometry. The cellular response was evaluated using HeLa cell attachment experiments for up to 24 hours. We expect that the coating platform established in this study will be translated into a number of biomedical applications, including applications in implantable devices and regenerative medicine.

Paper Details

Date Published: 23 December 2011
PDF: 9 pages
Proc. SPIE 8204, Smart Nano-Micro Materials and Devices, 82043T (23 December 2011); doi: 10.1117/12.910456
Show Author Affiliations
Peter Koegler, Commonwealth Scientific and Industrial Research Organisation (Australia)
Reutlingen Univ. (Germany)
Paul Pasic, Commonwealth Scientific and Industrial Research Organisation (Australia)
Graham Johnson, Commonwealth Scientific and Industrial Research Organisation (Australia)
Penny Bean, Commonwealth Scientific and Industrial Research Organisation (Australia)
Guenter Lorenz, Reutlingen Univ. (Germany)
Laurence Meagher, Commonwealth Scientific and Industrial Research Organisation (Australia)
Helmut Thissen, Commonwealth Scientific and Industrial Research Organisation (Australia)

Published in SPIE Proceedings Vol. 8204:
Smart Nano-Micro Materials and Devices
Saulius Juodkazis; Min Gu, Editor(s)

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