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Proceedings Paper

Quantitative determination of the human breast milk macronutrients by near-infrared Raman spectroscopy
Author(s): Edlene do Carmo M. Motta; Renato A. Zângaro; Landulfo Silveira
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Paper Abstract

This work proposes the evaluation of the macronutrient constitution of human breast milk based on the spectral information provided by near-infrared Raman spectroscopy. Human breast milk (5 mL) from a subject was collected during the first two weeks of breastfeeding and stocked in -20°C freezer. Raman spectra were measured using a Raman spectrometer (830 nm excitation) coupled to a fiber based Raman probe. Spectra of human milk were dominated by bands of proteins, lipids and carbohydrates in the 600-1800 cm-1 spectral region. Raman spectroscopy revealed differences in the biochemical constitution of human milk depending on the time of breastfeeding startup. This technique could be employed to develop a classification routine for the milk in Human Milk Banking (HMB) depending on the nutritional facts.

Paper Details

Date Published: 3 February 2012
PDF: 7 pages
Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82291F (3 February 2012); doi: 10.1117/12.910438
Show Author Affiliations
Edlene do Carmo M. Motta, Univ. Camilo Castelo Branco (Brazil)
Renato A. Zângaro, Univ. Camilo Castelo Branco (Brazil)
Landulfo Silveira, Univ. Camilo Castelo Branco (Brazil)


Published in SPIE Proceedings Vol. 8229:
Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV
Gerard L. Coté; Robert J. Nordstrom, Editor(s)

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