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Proceedings Paper

Three dimensional refractive index imaging with differential interference contrast microscopy
Author(s): Htet Aung; Jared Buckley; Piotr Kostyk; Braulio Rodriguez; Shelley Phelan; M. Xu
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Paper Abstract

We report here a new approach based on an extension of the transport of the intensity equation for three dimensional refractive index imaging of a weak phase object from a series of images recorded by a differential interference contrast microscope at different focus (z-stack). Our method is first validated by imaging polystyrene spheres. We then apply this method to monitor in vivo apoptosis of human breast MCF7 epithelial cells. The potential applications are discussed at the end.

Paper Details

Date Published: 2 February 2012
PDF: 8 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82270G (2 February 2012); doi: 10.1117/12.910339
Show Author Affiliations
Htet Aung, Fairfield Univ. (United States)
Jared Buckley, Fairfield Univ. (United States)
Piotr Kostyk, Fairfield Univ. (United States)
Braulio Rodriguez, Fairfield Univ. (United States)
Shelley Phelan, Fairfield Univ. (United States)
M. Xu, Fairfield Univ. (United States)

Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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