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Proceedings Paper

Assessing human skin with diffuse reflectance spectroscopy and colorimetry
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Paper Abstract

Colorimetry has been used as an objective measure of perceived skin color by human eye to document and score physiological responses of the skin from external insults. CIE color space values (L*, a* and b*) are the most commonly used parameters to correlate visually perceived color attributes such as L* for pigment, a* for erythema, and b* for sallowness of the skin. In this study, we investigated the relation of Lab color scale to the amount of major skin chromophores (oxy-, deoxyhemoglobin and melanin) calculated from diffuse reflectance spectroscopy. Thirty two healthy human subjects with ages from 20 to 70 years old, skin types I-VI, were recruited for the study. DRS and colorimetry measurements were taken from the left and right cheeks, and on the right upper inner arm. The melanin content calculated from 630-700 nm range of DRS measurements was shown to correlate with the lightness of skin (L*) for most skin types. For subjects with medium-to-light complexion, melanin measured at the blue part spectrum and hemoglobin interfered on the relation of lightness of the skin color to the melanin content. The sallowness of the skin that is quantified by the melanin contribution at the blue part spectrum of DRS was found to be related to b* scale. This study demonstrates the importance of documenting skin color by assessing individual skin chromophores with diffuse reflectance spectroscopy, in comparison to colorimetry assessment.

Paper Details

Date Published: 9 February 2012
PDF: 5 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82070P (9 February 2012); doi: 10.1117/12.910206
Show Author Affiliations
InSeok Seo, Johnson & Johnson CPPW (United States)
Yang Liu, Johnson & Johnson CPPW (United States)
Paulo R. Bargo, Johnson & Johnson CPPW (United States)
Nikiforos Kollias, Johnson & Johnson CPPW (United States)


Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg; Melissa J. Suter; Brian Jet-Fei Wong; Justus F. Ilgner; Stephen Lam; Bodo E. Knudsen; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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