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Proceedings Paper

A study of a self diagnostic platform for the detection of A2 biomarker for Leishmania donovani
Author(s): Philip J. R. Roche; Maurice C-K. Cheung; Mohamed Najih; Laura-Isobel McCall; Ibrahim Fakih; Vamsy P. Chodavarapu; Brian Ward; Momar Ndao; Andrew G. Kirk
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Paper Abstract

Visceral leishmaniasis (L.donovani) is a protozoan infection that attacks mononuclear phagocytes and causes the liver and spleen damage that can cause death. The investigation presented is a proof of concept development applying a plasmonic diagnostic platform with simple microfluidic sample delivery and optical readout. An immune-assay method is applied to the quantification of A2 protein, a highly immunogenic biomarker for the pathogen. Quantification of A2 was performed in the ng/ml range, analysis by ELISA suggested that a limit of 0.1ng/ml of A2 is approximate to 1 pathogen per ml and the sensing system shows the potential to deliver a similar level of quantification. Significant reduction in assay complexity as further enzyme linked enhancement is not required when applying a plasmonic methodology to an immunoassay. The basic instrumentation required for a portable device and potential dual optical readout where both plasmonic and photoluminescent response are assessed and investigated including consideration of the application of the device to testing where non-literate communication of results is considered and issues of performance are addressed.

Paper Details

Date Published: 15 February 2012
PDF: 9 pages
Proc. SPIE 8229, Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV, 82290C (15 February 2012); doi: 10.1117/12.910146
Show Author Affiliations
Philip J. R. Roche, McGill Univ. (Canada)
Maurice C-K. Cheung, McGill Univ. (Canada)
Mohamed Najih, McGill Univ. (Canada)
Laura-Isobel McCall, Montreal General Hospital (Canada)
Ibrahim Fakih, McGill Univ. (Canada)
Vamsy P. Chodavarapu, McGill Univ. (Canada)
Brian Ward, Montreal General Hospital (Canada)
Momar Ndao, Montreal General Hospital (Canada)
Andrew G. Kirk, McGill Univ. (Canada)


Published in SPIE Proceedings Vol. 8229:
Optical Diagnostics and Sensing XII: Toward Point-of-Care Diagnostics; and Design and Performance Validation of Phantoms Used in Conjunction with Optical Measurement of Tissue IV
Gerard L. Coté; Robert J. Nordstrom, Editor(s)

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