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Proceedings Paper

Multiplexed fluorescence detection for point of care
Author(s): Li Shen; Mike Ratterman; Tyler Stites; David Klotzkin; Ian Papautsky
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Paper Abstract

We report on the development of a portable fluorescence detection system. By combining a CMOS sensor and crosspolarization scheme, we achieved multiplexed detection with a single white emission LED excitation. We demonstrated fluorescence detection of Fluorescein and Rhodamine B in PDMS channels and achieved 1μM limit of detection (LOD). Microparticles with green and red fluorescence were detected simultaneously without changing the light sources or filters. We were able to clearly resolve microparticles, even if aggregated. The compact microfluorescence approach offers high spatial and spectral resolution, and is suitable for multiplexed detection in point-of-care applications.

Paper Details

Date Published: 14 February 2012
PDF: 7 pages
Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510S (14 February 2012); doi: 10.1117/12.910061
Show Author Affiliations
Li Shen, Univ. of Cincinnati (United States)
Mike Ratterman, Univ. of Cincinnati (United States)
Tyler Stites, Univ. of Cincinnati (United States)
David Klotzkin, Binghamton Univ. (United States)
Ian Papautsky, Univ. of Cincinnati (United States)

Published in SPIE Proceedings Vol. 8251:
Microfluidics, BioMEMS, and Medical Microsystems X
Holger Becker; Bonnie L. Gray, Editor(s)

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