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Proceedings Paper

Spiral inertial microfluidic devices for continuous blood cell separation
Author(s): Nivedita Nivedita; Phillip Ligrani; Ian Papautsky
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Paper Abstract

Enrichment and separation of cell components of blood is critical to clinical diagnostics and therapeutics. Here we report on spiral inertial microfluidic devices which achieve continuous size-based separation of cell mixtures with high throughput. These devices rely on hydrodynamic forces acting on cells within laminar flow, coupled with Dean instability-induced drag arising from the spiral microchannel geometry, to focus cells in streams near the inner channel wall. The spiral devices were optimized to achieve cell separation in less than 8 cm. These improved devices represent an important development because they are not only small in size (<1 in2), but exhibit high separation efficiency (~90%) and high throughput rates up to 1 million cells per minute. These device concepts offer a path towards possible development of a lab-on-chip for blood analysis and reagent free sample preparation, illustrated by the present results, which successfully demonstrate separation of erythrocytes from leukocytes with whole blood.

Paper Details

Date Published: 14 February 2012
PDF: 10 pages
Proc. SPIE 8251, Microfluidics, BioMEMS, and Medical Microsystems X, 82510R (14 February 2012); doi: 10.1117/12.909936
Show Author Affiliations
Nivedita Nivedita, Univ. of Cincinnati (United States)
Phillip Ligrani, Saint Louis Univ. (United States)
Ian Papautsky, Univ. of Cincinnati (United States)

Published in SPIE Proceedings Vol. 8251:
Microfluidics, BioMEMS, and Medical Microsystems X
Holger Becker; Bonnie L. Gray, Editor(s)

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