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Proceedings Paper

Development of a compact bio-optofluidic cell sorter
Author(s): Andrew Banas; Darwin Palima; Finn Pedersen; Jesper Glückstad
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Paper Abstract

We develop an active cell sorter that utilizes machine vision for cell identification. Particles are identified based on visual features such as shape, size and color using image processing. The sorter shares features from our previously developed BioPhotonics Workstation. Hence, it benefits from the extended axial manipulation range provided by the low numerical aperture geometry. Detected particles are catapulted axially by several hundred microns, allowing them to be moved from one laminar flow region to another. As the sorting motion is transverse to the viewing plane, multiple particles can be catapulted at the same time, therefore enabling parallel sorting. The sorter is developed with a minimal footprint such that it can operate as a table top device, an advantage over flow cytometry or FACS systems.

Paper Details

Date Published: 10 February 2012
PDF: 6 pages
Proc. SPIE 8274, Complex Light and Optical Forces VI, 82740N (10 February 2012); doi: 10.1117/12.909908
Show Author Affiliations
Andrew Banas, Technical Univ. of Denmark (Denmark)
Darwin Palima, Technical Univ. of Denmark (Denmark)
Finn Pedersen, Technical Univ. of Denmark (Denmark)
Jesper Glückstad, Technical Univ. of Denmark (Denmark)


Published in SPIE Proceedings Vol. 8274:
Complex Light and Optical Forces VI
Enrique J. Galvez; David L. Andrews; Jesper Glückstad; Marat S. Soskin, Editor(s)

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