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Proceedings Paper

Tri-modal confocal margin screening for the presence of residual squamous cell carcinoma in Mohs surgical excisions
Author(s): Anna Bar; Nicholas Snavely; Nathaniel Chen; Steven Jacques; Daniel S. Gareau
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Paper Abstract

Screening cancer in excision margins may be done with confocal microscopy to save time and cost over the gold standard histopathology (H&E). However, diagnostic accuracy requires sufficient contrast. Reflectance mode enables detection of large (>500um) nodular tumors. Enhanced nuclear contrast with acridine orange fluorescence mode additionally enables detection of tiny (<50um) basal cell carcinomas. Here, we present a novel combination of three modes to detect squamous cell carcinoma (SCC). Accurate screening of SCC requires eosin fluorescence, reflectance and acridine orange fluorescence to enable contrast for cytoplasm, collagen and nuclei respectively. Combining these signals replicates H&E for rapid clinical translation.

Paper Details

Date Published: 22 February 2012
PDF: 7 pages
Proc. SPIE 8214, Advanced Biomedical and Clinical Diagnostic Systems X, 821416 (22 February 2012); doi: 10.1117/12.909902
Show Author Affiliations
Anna Bar, Oregon Health & Science Univ. (United States)
Nicholas Snavely, Oregon Health & Science Univ. (United States)
Nathaniel Chen, Oregon Health & Science Univ. (United States)
Steven Jacques, Oregon Health & Science Univ. (United States)
Daniel S. Gareau, Oregon Health & Science Univ. (United States)


Published in SPIE Proceedings Vol. 8214:
Advanced Biomedical and Clinical Diagnostic Systems X
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

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