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Proceedings Paper

Tissue imaging with a stigmatic mass microscope using laser desorption/ionization
Author(s): Kunio Awazu; Hisanao Hazama; Tomonori Hamanaka; Jun Aoki; Michisato Toyoda; Yasuhide Naito
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Paper Abstract

A novel stigmatic mass microscope using laser desorption/ionization and a multi-turn time-of-flight mass spectrometer, MULTUM-IMG, has been developed. Stigmatic ion images of crystal violet masked by a fine square mesh grid with a 12.7 μm pitch were clearly observed, and the estimated spatial resolution was about 3 μm in the linear mode with a 20-fold ion optical magnification. Tissue sections of a brain and eyes of a mouse stained with crystal violet and methylene blue were observed in the linear mode, and the stigmatic total ion images of crystal violet and methylene blue agreed well with the optical photomicrograph of the same sections. Especially, the fine structure in the cornea tissue was clearly observed with a spatial resolution in the range of micrometers. Although the total measurement time of the stigmatic ion image for the whole-eye section was about 59 minutes using a laser with a 10 Hz repetition rate, the measurement time could be reduced to about 35 s using a laser with a 1 kHz repetition rate and automation of measurements. The stigmatic mass microscope developed in this research should be suitable for high-spatial resolution and high-throughput imaging mass spectrometry for pathology, pharmacokinetics, and so on.

Paper Details

Date Published: 9 February 2012
PDF: 7 pages
Proc. SPIE 8225, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X, 82252E (9 February 2012); doi: 10.1117/12.909898
Show Author Affiliations
Kunio Awazu, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Hisanao Hazama, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Tomonori Hamanaka, Osaka Univ. (Japan)
Jun Aoki, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Michisato Toyoda, Osaka Univ. (Japan)
Japan Science and Technology Agency (Japan)
Yasuhide Naito, The Graduate School for the Creation of New Photonics Industries (Japan)
Japan Science and Technology Agency (Japan)


Published in SPIE Proceedings Vol. 8225:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues X
Daniel L. Farkas; Dan V. Nicolau; Robert C. Leif, Editor(s)

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