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Proceedings Paper

Determination of optimal glycerol concentration for optical tissue clearing
Author(s): Eungjun Youn; Taeyoon Son; Han-Sung Kim; Byungjo Jung
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Paper Abstract

The laser scattering in tissue is significant in diagnostic and therapeutic purposes of laser. Many studies have been conducted to minimize laser scattering in tissue and therefore, to maximize the clinical efficacy by enhancing photon density. Optical clearing agents (OCAs) have been employed for optical tissue clearing (OTC). This study was aimed to investigate the optimal concentration of an OCA, glycerol, in topical application,, so that it can be utilized for clinical diagnosis and therapy in dermatology. Glycerol was topically applied to avoid possible edema caused by dermal injection. The effect of OTC was quantitatively evaluated as a function of the concentration of glycerol with various methods. Optical methods such as optical coherence tomography (OCT) and an integrating sphere were used to assess the enhancement of light penetration depth and refractive index matching. In addition, a non-optical method, ultrasound scanner, was utilized to evaluate quantitatively collagen dissociation. The results revealed that 70 % glycerol was the optimal concentration of OTC for topical application. This study may provide a guideline regarding to the use of glycerol for optimal diagnostic and therapeutic effects in dermatology.

Paper Details

Date Published: 9 February 2012
PDF: 8 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82070J (9 February 2012); doi: 10.1117/12.909790
Show Author Affiliations
Eungjun Youn, Yonsei Univ. (Korea, Republic of)
Taeyoon Son, Yonsei Univ. (Korea, Republic of)
Han-Sung Kim, Yonsei Univ. (Korea, Republic of)
Byungjo Jung, Yonsei Univ. (Korea, Republic of)

Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory M.D.; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg M.D.; Melissa J. Suter; Brian Jet-Fei Wong M.D.; Justus F. Ilgner M.D.; Stephen Lam; Bodo E. Knudsen M.D.; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney M.D.; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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