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Proceedings Paper

Mosaicing for fast wide-field-of-view optical resolution photoacoustic microscopy
Author(s): Peng Shao; Wei Shi; Ryan K. Chee; Alexander Forbrich; Roger J. Zemp
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Paper Abstract

The acquisition speed of previously reported mechanically-scanned Optical-Resolution Photoacoustic Microscopy (OR-PAM) systems has been limited by both laser pulse repetition rate and mechanical scanning speed. In this paper we introduce a mosaicing scheme wherein a grid of small sub-mm-scale field-of-view (FOV) patches are acquired in 0.5s per patch, and a 3-axis stepper-motor system is used to mechanically move the object to be imaged from patch-to-patch in less than 0.5s. Patch images are aligned and stitched to generate a large FOV image composite. This system retains the SNR-advantages of focused-transducer OR-PAM systems, and is a hybrid approach between optical-scanning and mechanical scanning. With this strategy we reduce the data acquisition time of previously reported large-FOV systems by a factor of around 23. SCID hairless mice are imaged. The wide-FOV, high-speed data acquisition OR-PAM system broadens the potential applications of the imaging modality.

Paper Details

Date Published: 23 February 2012
PDF: 9 pages
Proc. SPIE 8223, Photons Plus Ultrasound: Imaging and Sensing 2012, 822311 (23 February 2012); doi: 10.1117/12.909764
Show Author Affiliations
Peng Shao, Univ. of Alberta (Canada)
Wei Shi, Univ. of Alberta (Canada)
Ryan K. Chee, Univ. of Alberta (Canada)
Alexander Forbrich, Univ. of Alberta (Canada)
Roger J. Zemp, Univ. of Alberta (Canada)

Published in SPIE Proceedings Vol. 8223:
Photons Plus Ultrasound: Imaging and Sensing 2012
Alexander A. Oraevsky; Lihong V. Wang, Editor(s)

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