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Proceedings Paper

Critical considerations of pupil alignment to achieve open-loop control of MEMS deformable mirror in nonlinear laser scanning fluorescence microscopy
Author(s): Wei Sun; Yang Lu; Jason B. Stewart; Thomas G. Bifano; Charles P. Lin
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Paper Abstract

In this paper we present an alignment methodology for a non-linear laser scanning fluorescence microscopic imaging system integrated with a MEMS deformable mirror that is used to compensate microscope aberrations and improve sample image quality. The procedure uses an accurate open-loop control mechanism of the MEMS DM, a high resolution CMOS camera and a compact Shack-Hartmann wavefront sensor. The success of the indirect AO control method used by the microscope to compensate aberrations requires careful alignment of the optical system, specifically the DM conjugate planes in the scanning laser optical path. Considerations of this procedure are presented here, in addition to an assessment of the final accuracy of the alignment task is presented, by verifying the pupil conjugation and wavefront response. This method can also serve as a regular check-up of the system's performance and trouble-shoot for system misalignment.

Paper Details

Date Published: 15 February 2012
PDF: 7 pages
Proc. SPIE 8253, MEMS Adaptive Optics VI, 82530H (15 February 2012); doi: 10.1117/12.909652
Show Author Affiliations
Wei Sun, Wellman Ctr. for Photomedicine (United States)
Massachusetts General Hospital (United States)
Boston Univ. (United States)
Yang Lu, Boston Univ. (United States)
Jason B. Stewart, MIT Lincoln Lab. (United States)
Thomas G. Bifano, Boston Univ. (United States)
Charles P. Lin, Wellman Ctr. for Photomedicine (United States)
Massachusetts General Hospital (United States)
Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 8253:
MEMS Adaptive Optics VI
Scot S. Olivier; Thomas G. Bifano; Joel Kubby, Editor(s)

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