Share Email Print
cover

Proceedings Paper

Three-photon fluorescence imaging of melanin with a dual-wedge confocal scanning system
Author(s): Yair Mega; Joseph Kerimo; Joseph Robinson; Ali Vakili; Nicolette Johnson; Charles DiMarzio
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

Confocal microscopy can be used as a practical tool in non-invasive applications in medical diagnostics and evaluation. In particular, it is being used for the early detection of skin cancer to identify pathological cellular components and, potentially, replace conventional biopsies. The detection of melanin and its spatial location and distribution plays a crucial role in the detection and evaluation of skin cancer. Our previous work has shown that the visible emission from melanin is strong and can be easily observed with a near-infrared CW laser using low power. This is due to a unique step-wise, (SW) three-photon excitation of melanin. This paper shows that the same SW, 3-photon fluorescence can also be achieved with an inexpensive, continuous-wave laser using a dual-prism scanning system. This demonstrates that the technology could be integrated into a portable confocal microscope for clinical applications. The results presented here are in agreement with images obtained with the larger and more expensive femtosecond laser system used earlier.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822637 (9 February 2012); doi: 10.1117/12.909534
Show Author Affiliations
Yair Mega, The Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging Systems, Northeastern Univ. (United States)
Joseph Kerimo, The Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging Systems, Northeastern Univ. (United States)
Joseph Robinson, The Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging Systems, Northeastern Univ. (United States)
Ali Vakili, The Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging Systems, Northeastern Univ. (United States)
Nicolette Johnson, The Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging Systems, Northeastern Univ. (United States)
Univ. of Iowa (United States)
Charles DiMarzio, The Bernard M. Gordon Ctr. for Subsurface Sensing and Imaging Systems, Northeastern Univ. (United States)


Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

© SPIE. Terms of Use
Back to Top