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Proceedings Paper

High-speed spectral tuning CARS microscopy using AOTF laser
Author(s): Mamoru Hashimoto; Junichi Iwatsuka; Hirohiko Niioka; Tsutomu Araki
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Paper Abstract

We have developed a high speed spectral tuning CARS microscopy system using a mode-locked Ti:Sapphire laser with an acousto-optic tunable filter (AOTF) in the cavity. Since the wavelength of the laser is tunable with the applied radio frequency to the AOTF, the wavelength is electrically tunable.The pulse duration of the laser is about 10 ps, tunable range is 800 nm to 930 nm, and the tuning speed is ms order. The laser is synchronized with another mode-locked Ti:Sapphire laser laser our own method using a balance cross-correlator and phase lock loop technique. The synchronized lasers are used for light source of multi-focus CARS microscopy system using a microlens array scanner, and the hyperspectral imaging of adipocyte cells is demonstrated.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82261N (9 February 2012); doi: 10.1117/12.909507
Show Author Affiliations
Mamoru Hashimoto, Osaka Univ. (Japan)
Junichi Iwatsuka, Osaka Univ. (Japan)
Hirohiko Niioka, Osaka Univ. (Japan)
Tsutomu Araki, Osaka Univ. (Japan)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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