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Proceedings Paper

A novel multimodal CARS miniaturized microscope
Author(s): Brett Smith; Majid Naji; Sangeeta Murugkar; Craig Brideau; Peter Stys; Hanan Anis
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Paper Abstract

We demonstrate the operation of a novel portable, fibre delivery miniaturized multimodal microscope (exoscope) for coherent anti-Stokes Raman scattering and two-photon excitation fluorescence imaging using a single Ti:sapphire femtosecond pulsed laser. This microscope features a large mode area photonic crystal fibre for light delivery, as well as biaxial scanning microelectromechanical system mirrors and custom miniaturized optics corrected for chromatic aberration. We demonstrate imaging of polystyrene beads, two photon excitation fluorescence beads in both forward and backward (epi) directions. This miniaturized exoscope will enable in-vivo imaging of rat spinal cord.

Paper Details

Date Published: 9 February 2012
PDF: 6 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 822629 (9 February 2012); doi: 10.1117/12.909494
Show Author Affiliations
Brett Smith, Univ. of Ottawa (Canada)
Majid Naji, Univ. of Ottawa (Canada)
Sangeeta Murugkar, Univ. of Ottawa (Canada)
Craig Brideau, Hotchkiss Brain Institute, Univ. of Ottawa (Canada)
Peter Stys, Hotchkiss Brain Institute, Univ. of Ottawa (Canada)
Hanan Anis, Univ. of Ottawa (Canada)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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