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Proceedings Paper

Strip mosaicing confocal microscopy for rapid imaging over large areas of excised tissue
Author(s): Sanjee Abeytunge; Yongbiao Li; Bjorg Larson; Gary Peterson; Ricardo Toledo-Crow; Milind Rajadhyaksha
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Paper Abstract

Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in fresh tissue, without the processing that is required for conventional pathology. Previously, basal cell carcinoma margins were detected by mosaicing of confocal images of 12 x 12 mm2 of excised tissue from Mohs surgery. This mosaicing took 9 minutes. Recently we reported the initial feasibility of a faster approach called "strip mosaicing" on 10 x 10 mm2 of tissue that was demonstrated in 3 minutes. In this paper we report further advances in instrumentation and software. Rapid mosaicing of confocal images on large areas of fresh tissue potentially offers a means to perform pathology at the bedside. Thus, strip mosaicing confocal microscopy may serve as an adjunct to pathology for imaging tumor margins to guide surgery.

Paper Details

Date Published: 28 February 2012
PDF: 16 pages
Proc. SPIE 8214, Advanced Biomedical and Clinical Diagnostic Systems X, 821412 (28 February 2012); doi: 10.1117/12.909416
Show Author Affiliations
Sanjee Abeytunge, Memorial Sloan-Kettering Cancer Ctr. (United States)
Yongbiao Li, Memorial Sloan-Kettering Cancer Ctr. (United States)
Bjorg Larson, Memorial Sloan-Kettering Cancer Ctr. (United States)
Gary Peterson, Memorial Sloan-Kettering Cancer Ctr. (United States)
Ricardo Toledo-Crow, Memorial Sloan-Kettering Cancer Ctr. (United States)
Milind Rajadhyaksha, Memorial Sloan-Kettering Cancer Ctr. (United States)


Published in SPIE Proceedings Vol. 8214:
Advanced Biomedical and Clinical Diagnostic Systems X
Tuan Vo-Dinh; Anita Mahadevan-Jansen; Warren S. Grundfest, Editor(s)

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