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Dynamic contrast enhanced-magnetic resonance imaging (DCE-MRI) of photodynamic therapy (PDT) outcome and associated changes in the blood-brain barrier following Pc 4-PDT of glioma in an athymic nude rat model
Author(s): Vaijayantee Belle; Ali Anka; Nathan Cross; Paul Thompson; Eric Mott; Rahul Sharma; Kayla Gray; Ruozhen Zhang; Yueshuo Xu; Jiayang Sun; Chris A. Flask; Nancy L. Oleinick; David Dean
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Paper Abstract

Introduction: Dynamic Contrast-Enhanced-Magnetic Resonance Imaging (DCE-MRI) appears to provide an unambiguous means of tracking the outcome of photodynamic therapy (PDT) of brain tumors with the photosensitizer Pc 4. The increase in Gd enhancement observed after Pc 4-PDT may be due to a temporary opening of the blood-brain-barrier which, as noted by others, may offer a therapeutic window. Methods: We injected 2.5 x 105 U87 cells into the brains of 9 athymic nude rats. After 8-9 days peri-tumor DCE-MRI images were acquired on a 7.0 T microMRI scanner before and after the administration of 150 μL Gd. DCE-MRI scans were repeated three times following Pc 4-PDT. Results: The average, normalized peak enhancement in the tumor region, approximately 30-90 seconds after Gd administration, was 1.31 times greater than baseline (0.03 Standard Error [SE]) prior to PDT and was 1.44 (0.02 SE) times baseline in the first Post-PDT scans (Day 11), a statistically significant (p ≈ 0.014, N=8) increase over the Pre- PDT scans, and was 1.38 (0.02 SE) times baseline in the second scans (Day 12), also a statistically significant (p ≈ 0.008, N=7) increase. Observations were mixed in the third Post-PDT scans (Day 13), averaging 1.29 (0.03 SE) times baseline (p ≈ 0.66, N=7). Overall a downward trend in enhancement was observed from the first to the third Post-PDT scans. Discussion: DCE-MRI may provide an unambiguous indication of brain tumor PDT outcome. The initial increase in DCE-MRI signal may correlate with a temporary, PDT-induced opening of the blood-brain-barrier, creating a potential therapeutic window.

Paper Details

Date Published: 9 February 2012
PDF: 9 pages
Proc. SPIE 8207, Photonic Therapeutics and Diagnostics VIII, 82074T (9 February 2012); doi: 10.1117/12.909280
Show Author Affiliations
Vaijayantee Belle, Case Western Reserve Univ. (United States)
Ali Anka, Case Western Reserve Univ. (United States)
Nathan Cross, Case Western Reserve Univ. (United States)
Paul Thompson, Case Western Reserve Univ. (United States)
Eric Mott, Case Western Reserve Univ. (United States)
Rahul Sharma, Case Western Reserve Univ. (United States)
Kayla Gray, Case Western Reserve Univ. (United States)
Ruozhen Zhang, Case Western Reserve Univ. (United States)
Yueshuo Xu, Case Western Reserve Univ. (United States)
Jiayang Sun, Case Western Reserve Univ. (United States)
Chris A. Flask, Case Western Reserve Univ. (United States)
Nancy L. Oleinick, Case Western Reserve Univ. (United States)
David Dean, Case Western Reserve Univ. (United States)

Published in SPIE Proceedings Vol. 8207:
Photonic Therapeutics and Diagnostics VIII
Anita Mahadevan-Jansen; Andreas Mandelis; Kenton W. Gregory M.D.; Nikiforos Kollias; Hyun Wook Kang; Henry Hirschberg M.D.; Melissa J. Suter; Brian Jet-Fei Wong M.D.; Justus F. Ilgner M.D.; Stephen Lam; Bodo E. Knudsen M.D.; Steen Madsen; E. Duco Jansen; Bernard Choi; Guillermo J. Tearney M.D.; Laura Marcu; Haishan Zeng; Matthew Brenner; Krzysztof Izdebski, Editor(s)

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