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Proceedings Paper

Fluorescent nanodiamonds as highly stable biomarker for endotoxin verification
Author(s): Thorsten Bergmann; Jan Michael Burg; Maria Lilholt; Ulf Maeder; Sebastian Beer; Denise Salzig; Mehrdad Ebrahimi; Peter Czermak; Martin Fiebich
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Paper Abstract

Fluorescent nanodiamonds (ND) provide advantageous properties as a fluorescent biomarker for in vitro and in vivo studies. The maximum fluorescence occurs around 700 nm, they do not show photobleaching or blinking and seem to be nontoxic. After a pretreatment with strong acid fluorescent ND can be functionalized and coupled to endotoxin. Endotoxin is a decay product of bacteria and causes strong immune reactions. Therefore endotoxin has to be removed for most applications. An effective removal procedure is membrane filtration. The endotoxin, coupled to fluorescent ND can be visualized by using confocal microscopy which allows the investigation of the separation mechanisms of the filtration process within the membranes.

Paper Details

Date Published: 2 February 2012
PDF: 8 pages
Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82331H (2 February 2012); doi: 10.1117/12.909251
Show Author Affiliations
Thorsten Bergmann, Technische Hochschule Mittelhessen (Germany)
Jan Michael Burg, Technische Hochschule Mittelhessen (Germany)
Maria Lilholt, Technische Hochschule Mittelhessen (Germany)
Ulf Maeder, Technische Hochschule Mittelhessen (Germany)
Sebastian Beer, Technische Hochschule Mittelhessen (Germany)
Denise Salzig, Technische Hochschule Mittelhessen (Germany)
Mehrdad Ebrahimi, Technische Hochschule Mittelhessen (Germany)
Peter Czermak, Technische Hochschule Mittelhessen (Germany)
Kansas State Univ. (United States)
Martin Fiebich, Technische Hochschule Mittelhessen (Germany)


Published in SPIE Proceedings Vol. 8233:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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