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Proceedings Paper

Polymer-coated surface enhanced Raman scattering (SERS) gold nanoparticles for multiplexed labeling of chronic lymphocytic leukemia cells
Author(s): Christina M. MacLaughlin; Edward P. K. Parker; Gilbert C. Walker; Chen Wang
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Paper Abstract

The ease and flexibility of functionalization and inherent light scattering properties of plasmonic nanoparticles make them suitable contrast agents for measurement of cell surface markers. Immunophenotyping of lymphoproliferative disorders is traditionally undertaken using fluorescence detection methods which have a number of limitations. Herein, surface-enhanced Raman scattering (SERS) gold nanoparticles conjugated to monoclonal antibodies are used for the selective targeting of CD molecules on the surface of chronic lymphocytic leukemia (CLL) cells. Raman-active reporters were physisorbed on to the surface of 60 nm spherical Au nanoparticles, the particles were coated with 5kDa polyethylene glycol (PEG) including functionalities for conjugation to monoclonal IgG1 antibodies. A novel method for quantifying the number of antibodies bound to SERS probes on an individual basis as opposed to obtaining averages from solution was demonstrated using metal dots in transmission electron microscopy (TEM). The specificity of the interaction between SERS probes and surface CD molecules of CLL cells was assessed using Raman spectroscopy and dark field microscopy. An in-depth study of SERS probe targeting to B lymphocyte marker CD20 was undertaken, and proof-of-concept targeting using different SERS nanoparticle dyes specific for cell surface CD19, CD45 and CD5 demonstrated using SERS spectroscopy.

Paper Details

Date Published: 31 January 2012
PDF: 11 pages
Proc. SPIE 8212, Frontiers in Biological Detection: From Nanosensors to Systems IV, 821203 (31 January 2012); doi: 10.1117/12.909192
Show Author Affiliations
Christina M. MacLaughlin, Univ. of Toronto (Canada)
Edward P. K. Parker, Mount Sinai Hospital (Canada)
Gilbert C. Walker, Univ. of Toronto (Canada)
Chen Wang, Mount Sinai Hospital (Canada)

Published in SPIE Proceedings Vol. 8212:
Frontiers in Biological Detection: From Nanosensors to Systems IV
Benjamin L. Miller; Philippe M. Fauchet, Editor(s)

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