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Proceedings Paper

Use of the second harmonic generation microscopy to evaluate chondrogenic differentiation of mesenchymal stem cells for cartilage repair
Author(s): P. Bordeaux-Rego; M. O. Baratti; A. S. S. Duarte; T. B. Ribeiro; M. F. Andreoli-Risso; B. Vidal; J. B. Miranda; J. Adur; A. A. de Thomaz; V. B. Pelegati; F. F. Costa; H. F. Carvalho; C. L. Cesar; A. Luzo; S. T. Olalla Saad
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Paper Abstract

Articular cartilage injury remains one of the major concerns in orthopedic surgery. Mesenchymal stem cell (MSC) transplantation has been introduced to avoid some of the side effects and complications of current techniques.. With the aim to evaluate chondrogenic differentiation of mesenchymal stem cells, we used Second Harmonic Generation (SHG) microscopy to analyze the aggregation and orientation of collagen fibrils in the hyaline cartilage of rabbit knees. The experiment was performed using implants with type II collagen hydrogel (a biomaterial that mimics the microenvironment of the cartilage), one implant containing MSC and one other without MSC (control). After 10 weeks, the rabbit knees were dissected and fibril collagen distribution and spatial organization in the extracellular matrix of the lesions were verified by SHG. The result showed significant differences, whereas in histological sections of the cartilaginous lesions with MSC the collagen fibers are organized and regular; in the control sections the collagen fibers are more irregular, with absence of cells. A macroscopic analysis of the lesions confirmed this difference, showing a greater percentage of lesions filling in knees treated with MSC than in the knees used as controls. This study demonstrates that SHG microscopy will be an excellent tool to help in the evaluation of the effectiveness of MSC-based cell therapy for cartilage repair.

Paper Details

Date Published: 9 February 2012
PDF: 9 pages
Proc. SPIE 8226, Multiphoton Microscopy in the Biomedical Sciences XII, 82263N (9 February 2012); doi: 10.1117/12.909185
Show Author Affiliations
P. Bordeaux-Rego, Univ. Estadual de Campinas (Brazil)
M. O. Baratti, Univ. Estadual de Campinas (Brazil)
A. S. S. Duarte, Univ. Estadual de Campinas (Brazil)
T. B. Ribeiro, Univ. Estadual de Campinas (Brazil)
M. F. Andreoli-Risso, Univ. Estadual de Campinas (Brazil)
B. Vidal, Univ. Estadual de Campinas (Brazil)
J. B. Miranda, Univ. Estadual de Campinas (Brazil)
J. Adur, Univ. Estadual de Campinas (Brazil)
National Univ. of Entre Rios (Argentina)
A. A. de Thomaz, Univ. Estadual de Campinas (Brazil)
V. B. Pelegati, Univ. Estadual de Campinas (Brazil)
F. F. Costa, Univ. Estadual de Campinas (Brazil)
H. F. Carvalho, Univ. Estadual de Campinas (Brazil)
C. L. Cesar, Univ. Estadual de Campinas (Brazil)
A. Luzo, Univ. Estadual de Campinas (Brazil)
S. T. Olalla Saad, Univ. Estadual de Campinas (Brazil)

Published in SPIE Proceedings Vol. 8226:
Multiphoton Microscopy in the Biomedical Sciences XII
Karsten König, Editor(s)

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