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Proceedings Paper

Lipid-encapsulation of surface enhanced Raman scattering (SERS) nanoparticles and targeting to chronic lymphocytic leukemia (CLL) cells
Author(s): Shell Y. Ip; Christina M. MacLaughlin; Nisa Mullaithilaga; Michelle Joseph; Samantha Wala; Chen Wang; Gilbert C. Walker
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Paper Abstract

60 nm diameter gold nanoparticles (AuNP) were coated with a ternary mixture of lipids and targeted to human lymphocytes. Previously, the versatility, stability and ease of application of the lipid coating was demonstrated by the incorporation of three classes of Raman-active species. In the present study, lipid encapsulated AuNPs were conjugated to two targeting species, namely whole antibodies and antibody fragments (Fab), by two methods. Furthermore, in vitro targeting of lipid-encapsulated Au nanoparticles to patient-derived chronic lymphocytic leukemia (CLL) cells was demonstrated by Raman spectroscopy, Raman mapping, and darkfield microscopy. These results further demonstrate the versatility of the lipid layer for imparting stability, SERS activity, and targeting capability, which make these particles promising candidates for biodiagnostics.

Paper Details

Date Published: 31 January 2012
PDF: 12 pages
Proc. SPIE 8212, Frontiers in Biological Detection: From Nanosensors to Systems IV, 821204 (31 January 2012); doi: 10.1117/12.909179
Show Author Affiliations
Shell Y. Ip, Univ. of Toronto (Canada)
Christina M. MacLaughlin, Univ. of Toronto (Canada)
Nisa Mullaithilaga, Mount Sinai Hospital (Canada)
Michelle Joseph, Univ. of Toronto (Canada)
Samantha Wala, Mount Sinai Hospital (Canada)
Chen Wang, Mount Sinai Hospital (Canada)
Gilbert C. Walker, Univ. of Toronto (Canada)

Published in SPIE Proceedings Vol. 8212:
Frontiers in Biological Detection: From Nanosensors to Systems IV
Benjamin L. Miller; Philippe M. Fauchet, Editor(s)

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