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Proceedings Paper

Comparison of analysis methods for fluorescence lifetime imaging
Author(s): Tavis Hall; Dustin A. Dorroh; S. Elizabeth Robertson; D. T. Schaafsma
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Paper Abstract

We compare various time-gated and discrete-time methods for extracting the decay constant from an exponential signal, typical of fluorescence lifetime (FLIM) instruments, as well as a host of other physical phenomena. Analytical methods are evaluated for accuracy and precision as well as for computational and sampling efficiency.

Paper Details

Date Published: 3 February 2012
PDF: 6 pages
Proc. SPIE 8227, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX, 82271Q (3 February 2012); doi: 10.1117/12.909143
Show Author Affiliations
Tavis Hall, California Optical Engineering, Inc. (United States)
Dustin A. Dorroh, California Optical Engineering, Inc. (United States)
S. Elizabeth Robertson, California Optical Engineering, Inc. (United States)
D. T. Schaafsma, California Optical Engineering, Inc. (United States)


Published in SPIE Proceedings Vol. 8227:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX
Jose-Angel Conchello; Carol J. Cogswell; Tony Wilson; Thomas G. Brown, Editor(s)

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