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Proceedings Paper

Diffusion analysis of one photosensitizer in bovine teeth using fluorescence optical imaging
Author(s): S. Montanha; S. Pratavieira; D. P. Jacomassi; A. N. S. Rastelli; V. S. Bagnato
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Paper Abstract

Some photosensitizers (PSs) used for PACT (Antimicrobial Photodynamic Therapy) show an affinity for bacterial walls and can be photo-activated to cause the desired damage. However, on dentine bacterias may be less susceptible to PACT as a result of limited penetration of the PS. The aim of this study was to evaluate the diffusion of one PS based on hematoporphyrin on dentine structures. Twelve bovine incisors were used. Class III cavities (3 x 3 x 1mm) were prepared on the mesial or distal surfaces using a diamond bur. Photogem® solution at 1 mg/mL (10 uL for each cavity) was used. The experimental Groups were divided according to thickness of dentine remaining and etched or no-etched before the PS application. The fluorescence excitation source was a VelScope® system. For image capture a scientific CCD color camera PixelFly® was coupled to VelScope. For image acquisition and processing, a computational routine was developed at Matlab®. Fick's Law was used to obtain the average diffusion coefficient of PS. Differences were found between all Groups. The longitudinal temporal diffusion was influenced by the different times, thickness and acid etching.

Paper Details

Date Published: 1 February 2012
PDF: 7 pages
Proc. SPIE 8208, Lasers in Dentistry XVIII, 82080Q (1 February 2012); doi: 10.1117/12.909136
Show Author Affiliations
S. Montanha, Univ. Estadual Paulista (Brazil)
S. Pratavieira, Univ. of São Paulo (Brazil)
D. P. Jacomassi, Univ. of São Paulo (Brazil)
A. N. S. Rastelli, Univ. of São Paulo (Brazil)
Univ. Estadual Paulista (Brazil)
V. S. Bagnato, Univ. of São Paulo (Brazil)

Published in SPIE Proceedings Vol. 8208:
Lasers in Dentistry XVIII
Peter Rechmann; Daniel Fried, Editor(s)

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