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Proceedings Paper

Fluorescence-enhanced optical tomography and nuclear imaging system for small animals
Author(s): I-Chih Tan; Yujie Lu; Chinmay Darne; John C. Rasmussen; Banghe Zhu; Ali Azhdarinia; Shikui Yan; Anne M. Smith; Eva M. Sevick-Muraca
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Paper Abstract

Near-infrared (NIR) fluorescence is an alternative modality for molecular imaging that has been demonstrated in animals and recently in humans. Fluorescence-enhanced optical tomography (FEOT) using continuous wave or frequency domain photon migration techniques could be used to provide quantitative molecular imaging in vivo if it could be validated against "gold-standard," nuclear imaging modalities, using dual-labeled imaging agents. Unfortunately, developed FEOT systems are not suitable for incorporation with CT/PET/SPECT scanners because they utilize benchtop devices and require a large footprint. In this work, we developed a miniaturized fluorescence imaging system installed in the gantry of the Siemens Inveon PET/CT scanner to enable NIR transillumination measurements. The system consists of a CCD camera equipped with NIR sensitive intensifier, a diode laser controlled by a single board compact controller, a 2-axis galvanometer, and RF circuit modules for homodyne detection of the phase and amplitude of fluorescence signals. The performance of the FEOT system was tested and characterized. A mouse-shaped solid phantom of uniform optical properties with a fluorescent inclusion was scanned using CT, and NIR fluorescence images at several projections were collected. The method of high-order approximation to the radioactive transfer equation was then used to reconstruct the optical images. Dual-labeled agents were also used on a tumor bearing mouse to validate the results of the FEOT against PET/CT image. The results showed that the location of the fluorophore obtained from the FEOT matches the location of tumor obtained from the PET/CT images. Besides validation of FEOT, this hybrid system could allow multimodal molecular imaging (FEOT/PET/CT) for small animal imaging.

Paper Details

Date Published: 29 February 2012
PDF: 6 pages
Proc. SPIE 8216, Multimodal Biomedical Imaging VII, 82160J (29 February 2012); doi: 10.1117/12.909113
Show Author Affiliations
I-Chih Tan, The Univ. of Texas Health Science Ctr. at Houston (United States)
Yujie Lu, The Univ. of Texas Health Science Ctr. at Houston (United States)
Chinmay Darne, The Univ. of Texas Health Science Ctr. at Houston (United States)
John C. Rasmussen, The Univ. of Texas Health Science Ctr. at Houston (United States)
Banghe Zhu, The Univ. of Texas Health Science Ctr. at Houston (United States)
Ali Azhdarinia, The Univ. of Texas Health Science Ctr. at Houston (United States)
Shikui Yan, Siemens Medical Solutions USA, Inc. (United States)
Anne M. Smith, Siemens Medical Solutions USA, Inc. (United States)
Eva M. Sevick-Muraca, The Univ. of Texas Health Science Ctr. at Houston (United States)


Published in SPIE Proceedings Vol. 8216:
Multimodal Biomedical Imaging VII
Fred S. Azar; Xavier Intes, Editor(s)

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