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Proceedings Paper

Fluorescence enhancements and spectral modifications near the cut-off frequency of plasmonic structure
Author(s): Kareem Elsayad; Alexander Urich; Maria Nemethova; John V. Small; Karl Unterrainer; Katrin G. Heinze
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Paper Abstract

The effect of a population of fluorophores coupling to weakly bound surface plasmons in dielectric/metal/dielectric structures is investigated for the purpose of fluorescence enhancement near interfaces and live cell fluorescence surface imaging. We show theoretically and experimentally that for sufficient fluorophore concentrations near such SPP supporting structures significant enhancements in the radiative emission intensity can be observed, with a spectral modification that can be correlated to the average separation of the fluorophores from the substrate. We will discuss the theory behind the effect and some experimental results on imaging labeled proteins in the focal adhesion sites of cells.

Paper Details

Date Published: 6 February 2012
PDF: 15 pages
Proc. SPIE 8234, Plasmonics in Biology and Medicine IX, 82340W (6 February 2012); doi: 10.1117/12.909084
Show Author Affiliations
Kareem Elsayad, Research Institute of Molecular Pathology (Austria)
Alexander Urich, Technische Univ. Wien (Austria)
Maria Nemethova, Institute of Molecular Biotechnology GmbH (Austria)
John V. Small, Institute of Molecular Biotechnology GmbH (Austria)
Karl Unterrainer, Technische Univ. Wien (Austria)
Katrin G. Heinze, Research Institute of Molecular Pathology (Austria)
Univ. of Würzburg (Germany)

Published in SPIE Proceedings Vol. 8234:
Plasmonics in Biology and Medicine IX
Tuan Vo-Dinh; Joseph R. Lakowicz, Editor(s)

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