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Proceedings Paper

Calibrating the imaging and therapy performance of magneto-fluorescent gold nanoshells for breast cancer
Author(s): Adam Dowell; Wenxue Chen; Nrusingh Biswal; Ciceron Ayala-Orozco; Mario Giuliano; Rachel Schiff; Naomi J. Halas; Amit Joshi
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Paper Abstract

Gold nanoshells with NIR plasmon resonance can be modified to simultaneously enhance conjugated NIR fluorescence dyes and T2 contrast of embedded iron-oxide nanoparticles, and molecularly targeted to breast and other cancers. We calibrated the theranostic performance of magneto-fluorescent nanoshells, and contrasted the performance of molecularly targeted and untargeted nanoshells for breast cancer therapy, employing MCF-7L and their HER2 overexpressing derivative MCF-7/HER2-18 breast cancer cells as in vitro model systems. Silica core gold nanoshells with plasmon resonance on ~810 nm were doped with NIR dye ICG and ~10 nm iron-oxide nanoparticles in a ~20 nm epilayer of silica. A subset of nanoshells was conjugated to antibodies targeting HER2. Cell viability with varying laser power levels in presence and absence of bare and HER2-targeted nanoshells was assessed by calcein and propidium iodide staining. For MCF-7L cells, increasing power resulted in increased cell death (F=5.63, p=0.0018), and bare nanoshells caused more cell death than HER2-targeted nanoshells or laser treatment alone (F=30.13, p<0.001). For MCF-7/HER2-18 cells, death was greater with HER2-targeted nanoshells and was independent of laser power. This study demonstrates the capability of magneto-fluorescent nanocomplexes for imaging and therapy of breast cancer cells, and the advantages of targeting receptors unique to cancer cells.

Paper Details

Date Published: 25 February 2012
PDF: 7 pages
Proc. SPIE 8233, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV, 82330W (25 February 2012); doi: 10.1117/12.909066
Show Author Affiliations
Adam Dowell, Baylor College of Medicine (United States)
Wenxue Chen, Baylor College of Medicine (United States)
Nrusingh Biswal, Baylor College of Medicine (United States)
Ciceron Ayala-Orozco, Baylor College of Medicine (United States)
Mario Giuliano, Baylor College of Medicine (United States)
Rachel Schiff, Baylor College of Medicine (United States)
Naomi J. Halas, Rice Univ. (United States)
Amit Joshi, Baylor College of Medicine (United States)


Published in SPIE Proceedings Vol. 8233:
Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications IV
Samuel Achilefu; Ramesh Raghavachari, Editor(s)

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